Thermo Fisher Scientific H3R2me2sK4me2 Polyclonal Antibody
다중 종에서 반응하는 Rabbit Polyclonal 항체로, Histone H3의 Sym-dimethyl Arg2 및 Dimethyl Lys4 변형을 인식합니다. WB, IHC, ICC/IF, ChIP, Dot blot 등 다양한 응용에 적합하며, 고순도 Affinity chromatography로 정제되어 안정적인 액상 형태로 제공됩니다.
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Thermo Fisher Scientific H3R2me2sK4me2 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (IHC) | 1:100 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| ChIP assay (ChIP) | 2–5 µg/1×10⁶ cells |
| Dot blot (DB) | 1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | C. elegans, Chicken, Fruit fly, Human, Mouse, Plant, Rat, Xenopus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Histone H3 [Sym-dimethyl Arg2, Dimethyl Lys4]. Prepared from whole rabbit serum immunized with synthetic dimethylated peptides surrounding Arginine 2 / Lysine 4 of human Histone H3.2. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.91 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 30% glycerol |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid freeze/thaw cycles. Centrifuge if not clear after standing at room temperature. Stable for several weeks at 4°C as undiluted liquid. Dilute only prior to immediate use.
Anti-Histone H3 [Sym-dimethyl Arg2, Dimethyl Lys4] reacts with human Histone H3.2. BLAST analysis suggests cross-reactivity with Human, Mouse, and C. elegans. Predicted to react with Rat, Chicken, Xenopus, Drosophila, and Plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.
Target Information
Histone H3 is a DNA-binding protein found in the chromatin of all eukaryotic cells. Together with four core histone proteins, it forms the nucleosome structure. Histones are central to transcription regulation, DNA repair, replication, and chromosomal stability. The N-terminal tail of histone H3 undergoes various post-translational modifications such as methylation, acetylation, and phosphorylation, influencing chromatin structure and gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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