Thermo Fisher Scientific H3K36me2 Polyclonal Antibody
Histone H3의 Lys36 이중메틸화(H3K36me2)를 인식하는 Rabbit Polyclonal Antibody. WB, IHC, ICC/IF, ChIP, Dot blot 등 다양한 응용에 적합. 높은 특이성과 재현성 제공. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (IHC) | 1:500 |
| Immunocytochemistry (ICC/IF) | 1:500 |
| ChIP assay (ChIP) | 2–5 µg/1×10⁶ cells |
| Dot blot (DB) | 1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | C. elegans, Chicken, Fruit fly, Human, Mouse, Plant, Rat, Xenopus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Histone H3 [Dimethyl Lys36] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic dimethylated peptide surrounding Lysine 36 of human Histone H3.2. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.73 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | 0.02M potassium phosphate, pH 7.2, with 30% glycerol, 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at -20°C prior to opening.
- Aliquot contents and freeze at -20°C or below for extended storage.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
Anti-Histone H3 [Dimethyl Lys36] reacts with human Histone H3.2.
A BLAST analysis suggests cross-reactivity with human, mouse, and C. elegans.
Predicted to react with rat, chicken, Xenopus, Drosophila, and plant species based on 100% sequence homology.
Cross-reactivity with Histone H3 from other sources has not been determined.
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells.
H3, along with four core histone proteins, binds to DNA forming the nucleosome structure.
Histones play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability.
The N-terminal tail of histone H3 can undergo post-translational modifications such as methylation, acetylation, and phosphorylation, which influence chromatin structure and gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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