Thermo Fisher Scientific ATF6 Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

A suggested positive control is MCF7 cell lysate.
PA5-20215 can be used with blocking peptide PEP-0347.

Target Information

ATF6 is a transcription factor activated during endoplasmic reticulum (ER) stress, regulating unfolded protein response (UPR) target genes. It binds DNA on the ER stress response element (ERSE) and ERSE II, requiring NF-Y binding for activity.
ATF6 may also participate in transcriptional activation via serum response factor (SRF). It forms homodimers and heterodimers with ATF6 beta and interacts with NF-YC, GTF2I, YY1, and SRF.
Under ER stress, the cleaved N-terminal domain translocates into the nucleus, where the basic domain acts as a nuclear localization signal. The leucine-zipper domain mediates NF-Y binding and ERSE association.
During UPR, a ~50 kDa cytoplasmic fragment is released by sequential proteolysis via site-1 and site-2 proteases. ATF6 is N-glycosylated, phosphorylated by MAPK14/P38MAPK, and belongs to the bZIP family.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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