Thermo Fisher Scientific ATF6 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-1.0 µg/mL

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

5 µg/mL

-

Immunohistochemistry (PFA fixed) (IHC (PFA))

20 µg/mL

-

Immunocytochemistry (ICC/IF)

20 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A 17 amino acid peptide from near the amino terminus of human ATF6. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ATF6,uniProtId:P18850-1,ncbiNodeId:9606,antigenRange:1,antigenLength:670,antigenImageFileName:PA5-20216_ATF6_P18850-1_Rabbit.svg,antigenImageFileNamePDP:PA5-20216_ATF6_P18850-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11156398

Product Specific Information

A suggested positive control is MDA-MB-361 cell lysate.

PA5-20216 can be used with blocking peptide PEP-0349.

Target Information

ATF6 is a transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. It binds DNA on the 5-CCAC\[GA\]-3half of the ER stress response element (ERSE) (5-CCAAT-N(9)-CCAC\[GA\]-3) and of ERSE II (5-ATTGG-N-CCACG-3). Binding to ERSE requires binding of NF-Y to ERSE. ATF6 could also be involved in activation of transcription by the serum response factor. ATF6 exists as a homodimer and heterodimer with ATF6 beta. The dimer interacts with the nuclear transcription factor Y (NF-Y) trimer through direct binding to NF-Y subunit C (NF-YC). It Interacts also with the transcription factors GTF2I, YY1 and SRF. Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus. The basic domain of ATF6 functions as a nuclear localization signal and the basic leucine-zipper domain is sufficient for association with the NF-Y trimer and binding to ERSE. During the unfolded protein response an approximately 50 kDa fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 and site-2 proteases. ATF6 is N-glycosylated, phosphorylated in vitro by MAPK14/P38MAPK and belongs to the bZIP family.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.