Thermo Fisher Scientific IL-32 Polyclonal Antibody
Rabbit polyclonal antibody for detection of human IL-32. Validated for Western blot at 2.5–5 µg/mL. Supplied as liquid form in PBS with 0.02% sodium azide. Recommended positive control: Jurkat cell lysate. For research use only.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 2.5–5 µg/mL |
Product Specifications
| Category | Specification |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A 16 amino acid peptide from near the center of human IL-32 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Maintain refrigerated at 2–8°C for up to 3 months. For long-term storage, store at −20°C. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11153973 |
Product Specific Information
- Suggested positive control: Jurkat cell lysate
- PA5-20222 can be used with blocking peptide PEP-0335.
Target Information
Interleukin-32 (IL-32) was initially identified as a transcript (NK4) selectively expressed in lymphocytes and NK cells, with expression increased following activation by IL-2. It was later re-isolated from an IL-18-treated lung carcinoma cell line and renamed IL-32.
IL-32 is unique in that it does not share sequence homology with known cytokine families. It is highly expressed in immune tissues and exists in at least four differentially spliced isoforms. Treatment of human monocytic and mouse macrophage cells with IL-32 induces several proinflammatory cytokines such as TNF-α, IL-8, and MIP-2. IL-32 is also induced in human peripheral lymphocyte cells after mitogen stimulation and in epithelial cells by IFN-γ. These findings suggest that IL-32 may play a role in autoimmune and inflammatory diseases such as rheumatoid arthritis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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