Thermo Fisher Scientific Ki-67 (Proliferating Cell Marker) Monoclonal Antibody (rMKI67/6499)
인간 Ki-67 세포 증식 마커를 인식하는 마우스 단클론 항체로, IHC(P) 및 IHC(PFA)에서 사용 가능. 고순도 Protein A/G 정제, PBS(pH 7.4) 용액 형태, 보존제 무첨가. 세포주기 단계별 증식 연구용으로 적합.
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Thermo Fisher Scientific Ki-67 (Proliferating Cell Marker) Monoclonal Antibody (rMKI67/6499)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 2 µg/mL |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | rMKI67/6499 |
| Immunogen | Recombinant human Ki67 protein fragment (around aa 2293–2478) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
Target Information
Ki-67 is a nuclear protein expressed during various stages of the cell cycle, particularly during late G1, S, G2, and M phases. It contains a forkhead-associated (FHA) domain that mediates association with euchromatin at the perichromosomal layer, centromeric heterochromatin, and the nucleolus.
Ki-67 shows cell cycle-dependent localization: perinucleolar at G1, nuclear matrix-associated at G2, and chromosome-associated during M phase.
As it is absent in G0 cells and increases from G1 through mitosis, Ki-67 serves as a reliable marker for cellular proliferation.
Ki-67 antibodies are valuable for determining the growth fraction in neoplasms, with prognostic relevance comparable to the thymidine-labeling index. Low Ki-67 index correlates strongly with histologically low-grade tumors.
It is routinely used as a neuronal marker for cell cycling and proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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