Thermo Fisher Scientific Phospho-CHOP (Ser30) Polyclonal Antibody
Phospho-CHOP (Ser30) 폴리클로날 항체로, 인산화된 DDIT3(Ser30) 검출에 특이적입니다. Western blot, IHC, ICC/IF에 적합하며 인간, 마우스, 랫트 반응성을 가집니다. 고순도 정제, 액상 형태로 -20°C 보관.
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Thermo Fisher Scientific Phospho-CHOP (Ser30) Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human DDIT3 (Accession P35638), corresponding to amino acid residues around phosphorylated Ser30 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816352 |
Product Specific Information
Antibody detects endogenous levels of DDIT3 only when phosphorylated at Serine 30.
Target Information
GADD153 is a small nuclear protein capable of dimerizing with transcription factors C/EBP alpha and beta. The dimer inhibits normal C/EBP binding and function, while gaining activity toward non-classical C/EBP stress-related targets. Under normal conditions, GADD153 is not expressed at detectable levels but is highly upregulated during cellular or ER stress (e.g., tunicamycin treatment, nutrient starvation, or reducing agents affecting ER calcium flux).
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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