Thermo Fisher Scientific Phospho-HNF4A (Ser313) Polyclonal Antibody
HNF4A 단백질의 Ser313 인산화 형태를 특이적으로 검출하는 Rabbit Polyclonal Antibody. Western blot, IHC, ICC/IF 등 다양한 응용에 사용 가능. 인간, 생쥐, 랫드 반응성. 고순도 정제 및 안정한 액상 형태로 제공.
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Applications and Tested Dilution
| Application | Tested Dilution | References |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human HNF4A (Accession P41235), corresponding to amino acid residues around phosphorylated Ser313 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816363 |
Product Specific Information
Antibody detects endogenous levels of HNF4 alpha only when phosphorylated at Serine 313.
Target Information
Hepatocyte nuclear factor 4 alpha (HNF4α) is an orphan nuclear receptor expressed in the liver, kidney, intestine, and pancreas. Mutation of HNF4α in humans has been associated with maturity-onset diabetes of the young type 1 (MODY1). HNF4 binds to DNA as an exclusive homodimer. The HNF4α gene is alternatively spliced and may generate up to nine different isoforms, HNF4α1 through HNF4α9.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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