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Takara PicoPLEX Gold Single Cell DNA-Seq Kit
The PicoPLEX Gold Single Cell DNA-Seq Kit (PicoPLEX Gold) is designed to generate high-quality DNA libraries from single cells for sequencing on Illumina platforms.
This kit is based on Takara’s patented PicoPLEX technology, optimized for reproducible detection of:
- Copy number variants (CNVs)
- Single nucleotide variants (SNVs)
- Indels
- Small structural variants
The PicoPLEX Gold kit outperforms traditional Multiple Displacement Amplification (MDA) technology for SNV detection.
Key Features
- High-quality DNA library generation from single cells
- Optimized for CNV, SNV, indel, and structural variant detection
- Simple four-step workflow completed in under three hours
- Supports both fixed and unfixed single cells
- High reproducibility and uniform sequencing coverage
Technology Overview
PicoPLEX WGA (Whole Genome Amplification) uses quasi-random priming technology, recognized as the international gold standard for CNV detection in single cells.
Originally developed for aneuploidy and CNV analysis, the chemistry has been enhanced (PicoPLEX Gold) to improve accuracy and resolution for SNVs and small structural variants through optimized enzymes, primers, and protocols.
Workflow Summary
- Cell lysis and DNA release
- Pre-amplification
- Amplification
- Library preparation for Illumina sequencing
Total workflow time: < 3 hours with minimal hands-on time.
Compatible Index Kits
DNA HT Dual Index Kits
Designed for use with ThruPLEX and PicoPLEX library prep kits for multiplexed sequencing on Illumina platforms.
| Feature | Description |
|---|---|
| Index Type | Illumina Nextera® XT v2 |
| Total Indexes | 384 dual indexes |
| Format | 4 barcoded index plates (Cat. # R400660–R400663) or 24 individual tubes (Cat. # R400664) |
| Usage | Each plate well: single use / Each tube: up to two uses |
DNA Unique Dual Index Kits
For multiplexing up to 96 samples using IDT for Illumina UD index sequences.
| Feature | Description |
|---|---|
| Index Type | Unique Dual (UD) |
| Total Indexes | 96 dual indexes |
| Format | 4 sets of 24 tubes (Cat. Nos. R400665–R400668) |
| Usage | Each tube: up to two uses |
DNA Single Index Kits
For multiplexing up to 12 samples using TruSeq LT set A index sequences.
| Feature | Description |
|---|---|
| Index Type | Single Index |
| Total Indexes | 12 single indexes |
| Format | 4 sets of 12 tubes (Cat. Nos. R400695, R400697) |
| Usage | Each tube: up to eight uses |
Ordering Information
- Product Name: PicoPLEX Gold Single Cell DNA-Seq Kit
- Platform Compatibility: Illumina
- Application: Single-cell sequencing, CNV/SNV/indel detection
- Compatible Index Kits: DNA HT Dual, DNA Unique Dual, DNA Single Index Kits
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