Thermo Fisher Scientific alpha-2c Adrenergic Receptor Monoclonal Antibody (N330A/80), APC
Alpha-2C 아드레날린 수용체를 특이적으로 인식하는 단클론 항체로, APC 형광 표지되어 세포 분석 및 면역형광 실험에 적합합니다. Western blot 및 ICC/IF에 사용 가능하며, 인간과 생쥐 시료에 반응합니다. 단백질 G 정제 및 보존제 무첨가 포뮬레이션입니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N330A/80 |
| Immunogen | Synthetic peptide amino acids 442–462 (QDFRRSFKHILFRRRRRGFRQ, cytoplasmic C-terminus) of human Alpha-2C adrenergic receptor |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932069 |
Additional Formats Available
- Unconjugated (MA5-27724)
- FITC (MA5-45616)
- PE (MA5-45618)
- PerCP (MA5-45617)
- Custom conjugation available upon request
Product Specific Information
- 1 µg/mL of MA5-45615 was sufficient for detection of Alpha-2C Adrenergic Receptor in 20 µg of COS cells transiently transfected with HA-tagged Alpha-lysate and assayed by colorimetric immunoblot using goat anti-mouse IgG:HRP as secondary antibody.
- Detects 50 kDa or larger (possibly due to dimerization).
- Does not cross-react with other adrenergic receptors.
- This antibody was formerly sold as clone S330A-80.
Target Information
Alpha-2-adrenergic receptors are members of the G protein-coupled receptor superfamily, including three highly homologous subtypes: alpha2A, alpha2B, and alpha2C. These receptors play a key role in regulating neurotransmitter release from sympathetic nerves and adrenergic neurons in the central nervous system.
In mouse studies, both alpha2A and alpha2C subtypes were required for normal presynaptic control of transmitter release. The alpha2A subtype inhibited release at high stimulation frequencies, while the alpha2C subtype modulated neurotransmission at lower activity levels.
The alpha2C gene encodes a subtype lacking introns in both coding and untranslated sequences.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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