Thermo Fisher Scientific EphB4 Monoclonal Antibody (3D7G8)
EphB4 단백질을 인식하는 Mouse Monoclonal Antibody (Clone 3D7G8). Western blot, IHC, ICC/IF, ELISA, IP 등 다양한 응용에 사용 가능. Human 반응성, Protein A 정제, PBS buffer에 0.1% sodium azide 포함. 연구용으로만 사용.
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Thermo Fisher Scientific EphB4 Monoclonal Antibody (3D7G8)
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–2 µg/mL | View 3 publications |
| Immunohistochemistry (IHC) | Assay-dependent | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | – | View 1 publication |
| Immunocytochemistry (ICC/IF) | 3–5 µg/mL | View 2 publications |
| Flow Cytometry (Flow) | – | View 1 publication |
| ELISA | Assay-dependent | – |
| Immunoprecipitation (IP) | Assay-dependent | View 1 publication |
| Peptide Array (Array) | – | View 1 publication |
| Miscellaneous PubMed (Misc) | – | View 2 publications |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Hamster, Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3D7G8 |
| Immunogen | Recombinant protein derived from the C-terminal region of the human EphB4 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2533303 |
Product Specific Information
Antibody 37-1800 has been validated for use in Western blot, immunohistochemistry, immunofluorescence, ELISA, and immunoprecipitation assays.
In Western blot, it detects a ~125 kDa protein corresponding to EPHB4 in MCF7, HT-29, and U2OS cell lysates.
Target Information
EPHB4 is a receptor tyrosine kinase belonging to the ephrin receptor family. Eph receptors play key roles in nervous system development, angiogenesis, and synapse formation and maturation.
Ephrins are divided into ephrin-A (EFNA) and ephrin-B (EFNB) classes based on their membrane anchoring mechanisms. Ephrin receptors are classified by extracellular domain similarity and ligand affinity.
EphB4 binds to ephrin-B2 and is essential for vascular development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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