Thermo Fisher Scientific EphA4 Monoclonal Antibody (4C8H5)
EphA4 수용체 단백질에 특이적인 Thermo Fisher Scientific의 단클론 항체. Western blot, IHC, IP 등 다양한 응용 가능. Human, Mouse, Chicken 반응성. Protein A 정제, PBS 버퍼, -20°C 보관. 교차반응 없음, 연구용 전용.
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Applications 및 Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–3 µg/mL | 23 publications |
| Immunohistochemistry (IHC) | 3–10 µg/mL | 2 publications |
| Immunocytochemistry (ICC/IF) | – | 2 publications |
| ELISA | Assay-dependent | – |
| Immunoprecipitation (IP) | 5 µg | 3 publications |
| Blocking Assay (BLOCK) | – | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chicken, Human, Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4C8H5 |
| Immunogen | A synthetic peptide derived from the C-terminus human EphA4 receptor protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2533301 |
Product Specific Information
This antibody is specific for the endogenous EphA4 receptor protein.
No cross-reactivity with EphA5 or EphA7 has been detected.
On western blots, this antibody identifies a band at approximately 110–120 kDa.
Reactivity has been confirmed with EphA4-transfected 293T cells and mouse brain lysates by western blot and immunoprecipitation, and on transfected 293 cells and mouse hippocampus by immunohistochemistry.
Target Information
EPHA4 belongs to the ephrin receptor subfamily of the protein tyrosine kinase family.
EPH and EPH-related receptors are implicated in developmental events, particularly in the nervous system.
Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and two fibronectin type III repeats.
The ephrin receptors are divided into two groups based on the similarity of their extracellular domain sequences and their affinities for ephrin-A and ephrin-B ligands.
EphA4 encodes a protein that binds ephrin-A ligands.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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