Thermo Fisher Scientific EphA4 Monoclonal Antibody (4C8H5)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

View 23 publications 23 publications

Immunohistochemistry (IHC)

3-10 µg/mL

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

-

View 2 publications 2 publications

ELISA (ELISA)

Assay-dependent

-

Immunoprecipitation (IP)

5 µg

View 3 publications 3 publications

Blocking Assay (BLOCK)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Chicken, Human, Mouse

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

4C8H5

Immunogen

A synthetic peptide derived from the C-terminus human EphA4 receptor protein. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:EphA4,uniProtId:P54764-1,ncbiNodeId:9606,antigenRange:986,antigenLength:986,antigenImageFileName:37-1600_EphA4_P54764-1_House_mouse.svg,antigenImageFileNamePDP:37-1600_EphA4_P54764-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533301

Product Specific Information

This antibody is specific for the endogenous EphA4 receptor protein. No cross-reactivity with EphA5 or EphA7 has been detected. On western blots, this antibody identifies a band at ~110-120 kDa. Reactivity has been confirmed with EphA4-transfected 293T cells and mouse brain lysates by western blot and immunoprecipitation and on transfected 293 cells and mouse hippocampus by immunohistochemistry.

Target Information

EPHA4 belongs to the ephrin receptor subfamily of the protein tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediated developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. EphA4 encodes a protein that binds ephrin-A ligands.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.