Thermo Fisher Scientific Fibronectin Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

-

Immunohistochemistry (IHC)

-

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:200

-

Immunohistochemistry (Frozen) (IHC (F))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100

View 1 publication 1 publication

ELISA (ELISA)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Pig

Published species

Chicken, Mouse, Pig, Sheep

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Highly purified, human fibronectin from plasma. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Fibronectin,uniProtId:P02751-15,ncbiNodeId:9606,antigenRange:1-2477,antigenLength:2477,antigenImageFileName:PA1-23693_Fibronectin_P02751-15_Rabbit.svg,antigenImageFileNamePDP:PA1-23693_Fibronectin_P02751-15_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 1% BSA

Contains

0.05% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_558935

Product Specific Information

This antibody does not cross-react with Fibrinogen or other serum proteins.

Prolonged fixation in buffered formalin may destroy the epitope. Auto/Zyme predigestion is recommended when using this antibody with formalin-fixed paraffin-embedded tissue sections. Suggested positive controls for this product are human tonsil, liver, skin or kidney.

Target Information

Fibronectin is a disulfide-bonded dimer with a molecular weight range of 230-250 kDa. In the extracellular matrix of several connective tissues and vessels, fibronectin is present as an insoluble protein that is extensively cross-linked by interchain disulfide bonds forming high molecular mass polymers. Fibronectin is most abundant during embryonic development and tissue remodeling. Fibronectin is also present at high concentrations as a soluble plasma protein. Fibronectin is organized as a linear series of repeating modules which form domains for interaction with fibronectin itself, other matrix components (e.g. collagen and heparin) and receptors on cells (e.g. integrins). Fibronectin is present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. Fibronectin has been implicated in carcinoma development in lung cancer. Further, Fibronectin expression is increased especially in non-small cell lung carcinoma. The adhesion of lung carcinoma cells to Fibronectin enhances tumorgenecity and confers resistance to apoptosis induced by standard chemotherapeutic agents. The gene encoding Fibronectin has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some Fibronectin variants has not been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.