Thermo Fisher Scientific KIT Monoclonal Antibody (OTI2C1D5), TrueMAB
Human KIT 단백질을 인식하는 Mouse monoclonal antibody (Clone OTI2C1D5). Western blot 및 IHC(P) 검증 완료. 동결건조 형태로 제공되며, 재구성 후 1 mg/mL 농도. Affinity chromatography로 정제되어 고순도 확보. 연구용으로 KIT 관련 신호전달 및 암 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2C1D5 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 546–976 of human KIT produced in SF9 cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
KIT (c-KIT) is a proto-oncogene and a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor).
KIT regulates the growth and activation of various hematopoietic and non-hematopoietic cells.
Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism.
Activation of the KIT receptor triggers multiple signaling pathways including Ras/ERK, PI3K, PLC-γ, and JAK/STAT, essential for hematopoiesis, stem cell maintenance, and gametogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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