Thermo Fisher Scientific CD199 (CCR9) Monoclonal Antibody (eBioCW-1.2 (CW-1.2)), PE, eBioscience

Thermo Fisher Scientific CD199 (CCR9) Monoclonal Antibody (eBioCW-1.2 (CW-1.2)), PE, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.5 µg/test
Publications: View 11 publications

Product Specifications

Additional Formats:

• APC
• eFluor 450
• FITC
• PE-Cyanine7
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The eBioCW-1.2 monoclonal antibody reacts with mouse CCR9 (CD199), the receptor for thymus-expressed chemokine (TECK). CCR9 is a member of the G protein-coupled receptor (GPCR) superfamily, involved in trafficking of T cell progenitors within the thymus.
Expression begins at the DN3 stage (CD4⁻CD8⁻CD25⁺CD44⁻), peaks at the DP stage (CD4⁺CD8⁺CD25⁻CD44⁻), and is down-regulated in committed CD4⁺ or CD8⁺ SP thymocytes. CCR9-deficient mice show mild impairment in thymocyte development.
In peripheral tissues, CCR9 is expressed on naive CD8⁺ T cells, but not on naive CD4⁺ T cells.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse thymocytes; ≤0.5 µg/test (100 µL final volume). Recommended to titrate for optimal performance.
Excitation: 488–561 nm
Emission: 578 nm
Laser Compatibility: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered

Target Information

The CCR9 protein is a member of the beta chemokine receptor family, predicted to be a seven transmembrane G protein-coupled receptor.
Chemokines and their receptors regulate thymocyte migration and maturation under normal and inflammatory conditions.
The specific ligand is CCL25, and expression differs between T lymphocytes of the small intestine and colon, suggesting a role in thymocyte recruitment and immune specialization in the gastrointestinal tract.
This gene is mapped to the chemokine receptor gene cluster region and has two alternatively spliced transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지