Thermo Fisher Scientific CD197 (CCR7) Monoclonal Antibody (4B12), PE, eBioscience
Mouse CCR7 (CD197) 인식 단일클론 항체로, PE 형광 표지되어 Flow Cytometry에 최적화됨. 면역세포 이동 및 림프구 분화 연구에 활용 가능. 4B12 클론, Rat IgG2a isotype, 0.2 mg/mL 농도, 4°C 암저장.
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Thermo Fisher Scientific CD197 (CCR7) Monoclonal Antibody (4B12), PE, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 1 µg/test | View 53 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), PE |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4B12 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_465904 |
Additional Formats Available
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- eFluor 450
- Functional Grade
- PE-Cyanine7
- PE-eFluor 610
- PerCP-Cyanine5.5
- Custom conjugation available
Product Specific Information
The 4B12 monoclonal antibody reacts with mouse CCR7 (CD197), a chemokine receptor for CCL19 and CCL21. CCR7 mediates lymphocyte migration to secondary lymphoid organs and plays roles in dendritic cell and B cell localization during immune responses.
Human CCR7 is a marker for central memory T cells (TCM) and participates in thymocyte development. Deficiency leads to disrupted thymic architecture and abnormal T cell development.
For optimal CCR7 visualization, multi-color staining and incubation at 37°C may be necessary. The 4B12 antibody shows similar staining patterns to polyclonal CCR7 antibodies and specifically binds CCR7-GFP fusion proteins.
Applications Reported
- Flow cytometric analysis of mouse splenocytes and thymocytes.
Applications Tested
- ≤1 µg/test (100 µL final volume, 10⁵–10⁸ cells/test).
- Titrate for optimal performance.
Important Staining Notes
- Staining at 37°C improves brightness and resolution.
- Co-staining with CCL19-Fc may be hindered due to steric effects.
Recommended Protocol
- Block FcγIII/II receptors with 5 µg/million cells anti-mouse CD16/32 for 15 min on ice.
- Add 1 µg/million cells 4B12 and incubate at 37°C or 2–8°C for 30 min.
- Wash once with Flow Cytometry Staining Buffer and decant.
- Analyze or proceed with secondary staining.
Technical Notes
- Excitation: 488–561 nm
- Emission: 578 nm
- Compatible Lasers: Blue, Green, Yellow-Green
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
CCR7 is a G protein-coupled chemokine receptor induced by Epstein-Barr virus (EBV). It is expressed in blood, bone marrow, lymph nodes, and intestine, especially in activated B and T lymphocytes. CCR7 regulates memory T cell migration and dendritic cell maturation. Its ligand CCL19 (ECL) specifically binds CCR7. The C-C chemokine receptor family (CCR1–CCR5, Duffy antigen) mediates T cell chemotaxis and phagocyte migration to inflammation sites.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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