Thermo Fisher Scientific CD197 (CCR7) Monoclonal Antibody (4B12), PE, eBioscience

Thermo Fisher Scientific CD197 (CCR7) Monoclonal Antibody (4B12), PE, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats Available

• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• eFluor 450
• Functional Grade
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Custom conjugation available

Product Specific Information

The 4B12 monoclonal antibody reacts with mouse CCR7 (CD197), a chemokine receptor for CCL19 and CCL21. CCR7 mediates lymphocyte migration to secondary lymphoid organs and plays roles in dendritic cell and B cell localization during immune responses.

Human CCR7 is a marker for central memory T cells (TCM) and participates in thymocyte development. Deficiency leads to disrupted thymic architecture and abnormal T cell development.

For optimal CCR7 visualization, multi-color staining and incubation at 37°C may be necessary. The 4B12 antibody shows similar staining patterns to polyclonal CCR7 antibodies and specifically binds CCR7-GFP fusion proteins.

Applications Reported

• Flow cytometric analysis of mouse splenocytes and thymocytes.

Applications Tested

• ≤1 µg/test (100 µL final volume, 10⁵–10⁸ cells/test).
• Titrate for optimal performance.

Important Staining Notes

• Staining at 37°C improves brightness and resolution.
• Co-staining with CCL19-Fc may be hindered due to steric effects.

Recommended Protocol

1. Block FcγIII/II receptors with 5 µg/million cells anti-mouse CD16/32 for 15 min on ice.
2. Add 1 µg/million cells 4B12 and incubate at 37°C or 2–8°C for 30 min.
3. Wash once with Flow Cytometry Staining Buffer and decant.
4. Analyze or proceed with secondary staining.

Technical Notes

• Excitation: 488–561 nm
• Emission: 578 nm
• Compatible Lasers: Blue, Green, Yellow-Green
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

CCR7 is a G protein-coupled chemokine receptor induced by Epstein-Barr virus (EBV). It is expressed in blood, bone marrow, lymph nodes, and intestine, especially in activated B and T lymphocytes. CCR7 regulates memory T cell migration and dendritic cell maturation. Its ligand CCL19 (ECL) specifically binds CCR7. The C-C chemokine receptor family (CCR1–CCR5, Duffy antigen) mediates T cell chemotaxis and phagocyte migration to inflammation sites.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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