Thermo Fisher Scientific Bax Recombinant Mouse Monoclonal Antibody (6A7)
Bax 단백질의 활성 형태를 특이적으로 인식하는 재조합 마우스 단일클론 항체. Western blot, IHC, IP 등 다양한 응용에 사용 가능. Human, Mouse, Rat 반응성. HEK293 세포 발현, Protein A 정제, 1 mg/mL 농도. 단기 4°C, 장기 -20°C 보관.
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- pk
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (IHC) | Assay-dependent |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 6A7 |
| Immunogen | KLH conjugated to peptides corresponding to amino acids 3–16 of murine Bax (CGSGEQLGSGGPTSS), amino acids 3–16 of human Bax (CGSGEQPRGGGPTSS), and amino acids 12–24 shared by Bax from both species (CGPTSSEQIMKTGA). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
Product Specific Information
In the presence of nonionic detergent, the antibody avidly binds the monomeric form of Bax, but not Bax complexed with either Bcl-XL or Bcl-2. The Bax (6A7) antibody only recognizes conformationally changed active Bax.
Target Information
BAX is a member of the Bcl-2 family and plays an important role in the regulation of apoptosis. Whereas Bcl-2 is commonly regarded as an anti-apoptotic protein, BAX functions as a pro-apoptotic regulator. Apoptosis regulation involves both homo- and heterodimerization of different isoforms of BAX and Bcl-2.
The Bax gene encodes different isoforms including Bax alpha (21 kDa) and Bax beta (24 kDa). Both isoforms contain BH1, BH2, and BH3 domains; however, Bax beta has a unique carboxyl terminus and lacks a hydrophobic transmembrane domain.
Bcl-2 also exists in multiple isoforms. Bcl-2 beta differs from variant alpha in the 3′ UTR and coding region, resulting in a shorter (22 kDa) form with a distinct C-terminus compared to Bcl-2 alpha (26 kDa). Overexpression of BAX promotes cell death, and BAX can form homodimers or heterodimers with other BCL-2 related proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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