Thermo Fisher Scientific Phospho-MEK1/MEK2 (Ser221) Monoclonal Antibody (K.742.1)
MEK1/MEK2의 Ser221 인산화 상태를 검출하는 단클론 항체로 Western blot, IHC, Flow cytometry에 적합. 다양한 종(인간, 마우스, 랫 등)에 반응하며 비결합형 액상 형태로 제공. 고순도의 친화 크로마토그래피 정제 제품.
- 판매단위
- pk
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | K.742.1 |
| Immunogen | Synthetic phosphopeptide corresponding to residues surrounding pSer221 of human MEK1/2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 246 µg/mL |
| Purification | Affinity chromatography |
| Storage buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol |
| Contains | <0.02% sodium azide |
| Storage conditions | -20°C |
| Shipping conditions | Wet ice |
| RRID | AB_10985371 |
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
MEK1 (MAP Kinase Kinase, also known as MKK) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation (Ahn, 1993; Chong et al., 2003). In brain, this pathway also plays a key role in synaptic signaling (Adams and Sweatt, 2002). Activated MEK1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase (Kyriakis et al., 1991; Seger et al., 1991; Crews et al., 1992).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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