Thermo Fisher Scientific ZAP70 (Chronic Lymphocytic Leukemia Marker) Monoclonal Antibody (ZAP70/2047)
ZAP70 단백질(Chronic Lymphocytic Leukemia Marker)을 인식하는 Mouse Monoclonal Antibody. 다양한 면역학적 응용(IHC, ICC/IF, Flow, ELISA)에 최적화. Human 시료 반응성, Protein A/G 정제, 4°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
| ELISA | 2–4 µg/mL |
| Peptide Array | Assay-dependent |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | ZAP70/2047 |
| Immunogen | Recombinant fragment (around aa 247–382) of human ZAP70 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
ZAP-70 is a cytosolic protein tyrosine kinase belonging to the Syk family. It is expressed exclusively in T-cells and natural killer cells and is essential for T-cell receptor activation. Upon TCR engagement, ZAP70 is phosphorylated on tyrosines 292, 315, and 319 in the interdomain B region, located between the SH2 and kinase domains. Phosphorylation of tyrosines 315 (Vav binding site) and 319 (Lck binding site) enhances ZAP70-mediated lymphocyte signaling, while tyrosine 292 phosphorylation terminates transient activation and attenuates signaling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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