Thermo Fisher Scientific ZAP70 (Chronic Lymphocytic Leukemia Marker) Monoclonal Antibody (ZAP70/2047)
ZAP70 단백질을 인식하는 인간 특이적 단클론 항체로, 만성 림프구성 백혈병(CLL) 마커 연구에 적합. IHC, ICC, Flow, ELISA 등 다양한 응용 가능. Recombinant fragment 기반으로 제작된 고순도 항체. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
| ELISA | 2–4 µg/mL |
| Peptide Array | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | ZAP70/2047 |
| Immunogen | Recombinant fragment (around aa 247–382) of human ZAP70 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | –20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
ZAP-70 is a cytosolic protein tyrosine kinase belonging to the Syk family. It is expressed exclusively in T-cells and natural killer cells and is essential for T-cell receptor activation. Upon TCR engagement, ZAP70 undergoes phosphorylation on tyrosines 292, 315, and 319 within the interdomain B region between the SH2 and kinase domains. Phosphorylation at tyrosines 315 (Vav binding site) and 319 (Lck binding site) enhances ZAP70’s role in lymphocyte signaling, while tyrosine 292 phosphorylation attenuates its activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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