Thermo Fisher Scientific ZAP70 (Chronic Lymphocytic Leukemia Marker) Monoclonal Antibody (rZAP70/2046)
인간 ZAP-70 단백질을 인식하는 Mouse monoclonal 항체로, 만성 림프구성 백혈병(CLL) 마커 연구에 적합합니다. IHC 및 ELISA에 사용 가능하며, 단백질 A/G로 정제된 액상 형태입니다. -20°C에서 보관하며, 보존제가 포함되어 있지 않습니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| ELISA | 2–4 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | rZAP70/2046 |
| Immunogen | Recombinant fragment (around aa 247–382) of human ZAP70 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
ZAP-70 is a cytosolic protein tyrosine kinase belonging to the Syk family. It is expressed exclusively in T-cells and natural killer cells and is required for T-cell receptor (TCR) activation.
Upon TCR engagement, ZAP70 is phosphorylated on tyrosines 292, 315, and 319 in the interdomain B region between the SH2 and kinase domains.
Phosphorylation of tyrosines 315 (Vav binding site) and 319 (Lck binding site) enhances ZAP70-mediated lymphocyte signaling, while phosphorylation at tyrosine 292 attenuates this activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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