Thermo Fisher Scientific TRRAP Polyclonal Antibody
TRRAP 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, Western blot, IP, ChIP 등 다양한 응용에 적합. 인간에 반응하며, 고순도 항원 친화 크로마토그래피 정제. 장기 보관 시 -20°C에서 안정적 유지.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunoprecipitation (IP) | 1:100–1:500 |
| ChIP assay (ChIP) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human TRRAP (exact sequence proprietary) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2736753 |
Product Specific Information
- Positive Control: Jurkat, Raji, NCI-H929, 293T, A431, HeLa, HepG2
- Predicted Reactivity: Mouse (98%), Rat (98%), Japanese Medaka (89%), Bovine (100%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
TRRAP is an adapter protein found in various multiprotein chromatin complexes with histone acetyltransferase activity (HAT), contributing to epigenetic transcription activation. It is a component of the NuA4 histone acetyltransferase complex responsible for acetylation of nucleosomal histones H4 and H2A.
TRRAP plays a central role in MYC transcription activation and participates in cell transformation by MYC. It is required for transcription activation mediated by p53/TP53, E2F1, E2F4, and adenovirus E1A.
TRRAP likely links transcription factors such as E1A, MYC, or E2F1 to HAT complexes like STAGA, facilitating transcription activation. It may also be involved in the mitotic checkpoint and normal cell cycle progression, and is a component of a SWR1-like complex that mediates removal of histone H2A.Z/H2AFZ from nucleosomes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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