Thermo Fisher Scientific BNIP3 Polyclonal Antibody, DyLight 550
BNIP3 단백질 검출용 DyLight 550 결합 폴리클로날 항체. Western blot 및 면역세포염색(ICC/IF)에 적합. 고감도 형광 검출(Ex/Em 556/570 nm). 인체 BNIP3 N-말단 펩타이드 기반 면역원. 연구용으로만 사용 가능.
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Thermo Fisher Scientific BNIP3 Polyclonal Antibody, DyLight 550
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide against an N-terminal region (within residues 1–50) of the human BNIP3 protein |
| Conjugate | DyLight™ 550 |
| Excitation / Emission Max | 556 / 570 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Wet ice |
| RRID | AB_11152224 |
Product Specific Information
In Western blot, a band is observed in hypoxic samples at approximately 30 and 60 kDa, representing the BNIP3 protein.
Non-specific bands may also be present.
It is recommended to run normoxic samples alongside hypoxic samples as a negative control.
Target Information
NIP3 is a member of the BCL2/adenovirus E1B 19 kDa-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein, which protects against virally induced cell death, and with BCL2-like sequences that also serve as apoptotic protectors.
NIP3 contains a BH3 domain and a transmembrane domain associated with pro-apoptotic function.
This dimeric mitochondrial protein induces apoptosis even in the presence of BCL2.
주의사항
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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