Thermo Fisher Scientific HDAC8 Monoclonal Antibody (OTI1A4), TrueMAB
HDAC8 단백질을 인식하는 Mouse IgG1 단일클론 항체로 Western blot에 적합. 인간, 마우스, 랫트 반응성. 무보존제, 동결건조 형태로 제공되며 1 mg/mL 농도. 크로마틴 구조 연구 및 히스톤 탈아세틸화 관련 연구에 활용 가능.
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Applications
- Western Blot (WB): 1:500–1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1A4 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1–243 of human HDAC8 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
In the intact cell, DNA interacts with histones and nuclear proteins to form chromatin. Chromatin remodeling, regulated by histone acetylation and deacetylation, is critical for transcriptional control.
Histone acetylation increases DNA accessibility to transcription factors, while deacetylation leads to transcriptional silencing.
HDAC8, a nuclear histone deacetylase isolated from human kidney, shares homology with other HDACs but exhibits distinct tissue distribution. It plays a role in tissue development and cancer etiology.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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