Thermo Fisher Scientific ICAM-1 Monoclonal Antibody (84H10)
Thermo Fisher Scientific의 ICAM-1 Monoclonal Antibody (84H10)는 인간 및 개 ICAM-1(CD54) 단백질을 인식하는 mouse IgG1 항체입니다. IHC, Flow Cytometry, ELISA, IP 등 다양한 응용에 적합하며, Protein A로 정제된 액상 형태로 제공됩니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (Frozen) (IHC (F)) | 1:20–1:50 | - |
| Flow Cytometry (Flow) | Neat–1:10 | 2 publications |
| ELISA (ELISA) | Assay-dependent | - |
| Immunoprecipitation (IP) | 5 µg × 10^7 cells | - |
| Functional Assay (Functional) | Assay-dependent | - |
| Miscellaneous PubMed (Misc) | - | 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 84H10 |
| Immunogen | K562 cell line (target: ICAM-1 (CD54), UniProt ID: P05362-1, antigen range: 1–532) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | <0.1% sodium azide |
| Storage Conditions | Store at 4°C short term; for long-term storage, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_929070 |
Product Specific Information
- Contains sodium azide; removal by dialysis is recommended prior to use in functional assays.
- For FACS analysis, use 10 µL of the suggested working dilution to label 1×10^6 cells in 100 µL.
- Mouse anti-Human CD54 antibody, clone 84H10 recognizes the D1 domain of ICAM-1.
Target Information
ICAM-1 (CD54) is an 85–110 kDa single-chain type I integral membrane glycoprotein composed of five immunoglobulin superfamily repeats, a transmembrane region, and a cytoplasmic domain.
It has seven potential N-linked glycosylation sites and shares sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102).
ICAM-1 binds to integrins CD11a/CD18 (LFA-1) and CD11b/CD18 (Mac-1), and serves as a receptor for Rhinovirus.
It is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B and T lymphocytes, monocytes, macrophages, dendritic cells, and neutrophils.
Expression increases upon inflammation and is also found in some carcinoma and melanoma cells.
Soluble ICAM-1 is detectable in plasma and elevated in patients with inflammatory syndromes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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