Thermo Fisher Scientific FDFT1 Monoclonal Antibody (OTI2F10), TrueMAB
인간 FDFT1 단백질을 인식하는 Mouse IgG2a 단일클론 항체. Western blot, IHC, ICC/IF에 사용 가능. 무보존제, 동결건조 형태로 제공되며, 재구성 및 결합 실험에 적합. 콜레스테롤 생합성 경로 연구용.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200–1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2F10 |
| Immunogen | Full-length human recombinant FDFT1 protein produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
FDFT1 catalyzes the first step in the cholesterol biosynthetic pathway, converting trans-farnesyldiphosphate to squalene.
Loss of promoter activity and sterol response for FDFT1 is localized to a 69-bp region positioned 131 bp upstream of the transcription start site. This region contains a sterol regulatory element-1 (SRE1) and two putative NF1 binding sites.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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