Thermo Fisher Scientific 6x-His Tag Monoclonal Antibody (HIS.H8), DyLight 680

Thermo Fisher Scientific 6x-His Tag Monoclonal Antibody (HIS.H8), DyLight 680

Applications and Tested Dilutions

Publications

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Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 555
• Alexa Fluor 647
• Biotin
• DyLight 488
• DyLight 550
• DyLight 650
• HRP
• Custom conjugation available upon request

Product Specific Information

MA1-21315-D680 has been successfully used in immunofluorescence and Western blot applications.
DyLight 680 exhibits excitation/emission maxima at 692/712 nm.

Target Information

The 6x His tag is a synthetic oligopeptide consisting of six consecutive histidine residues (HHHHHH).
It is commonly expressed at the N- or C-terminal of recombinant proteins to facilitate isolation or purification via immobilized metal affinity chromatography (IMAC).
Epitope tagging enables detection of proteins lacking specific antibodies by fusing a known epitope to the recombinant protein.
His-tagged proteins can be purified using Ni²⁺-NTA adsorbents due to strong and selective metal chelation by histidine clusters.
His tag-specific antibodies are used for detection or co-immunoprecipitation of tagged proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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