Thermo Fisher Scientific 6x-His Tag Monoclonal Antibody (HIS.H8), HRP
6x-His 태그 단백질 검출용 HRP 결합 단일클론 항체. Western blot, ELISA 등 다양한 응용에 적합. 높은 특이성과 재현성을 제공하며, 단백질 정제 및 검출 실험에 활용 가능. 연구용 전용 제품.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500 | View 31 publications |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| ELISA | - | View 3 publications |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Tag |
| Published Species | Tag |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | HIS.H8 |
| Immunogen | 6x His synthetic peptide |
| Conjugate | HRP |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.2, with proprietary stabilizer |
| Contains | 0.07% Kathon |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2536989 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 555
- Alexa Fluor 647
- Biotin
- DyLight 488
- DyLight 550
- DyLight 650
- DyLight 680
- Request custom conjugation
Target Information
The 6x His tag is a synthetic oligopeptide consisting of six consecutive histidine residues (HHHHHH). It is commonly expressed at either the N- or C-terminal regions of recombinant proteins to enable isolation or purification via immobilized metal affinity chromatography (IMAC).
Epitope tagging allows detection of proteins for which no specific antibody exists by fusing a known epitope to the recombinant protein. His-tagged proteins are purified based on the strong and selective affinity of histidine clusters to metal ions such as Ni²⁺, enabling purification to homogeneity using Ni²⁺-NTA adsorbents.
His tag-specific antibodies, such as HIS.H8, facilitate detection and co-immunoprecipitation of tagged proteins.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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