Thermo Fisher Scientific Malondialdehyde Monoclonal Antibody (6H6), PE
Malondialdehyde 단백질 결합체를 특이적으로 인식하는 PE 결합 단클론 항체입니다. Western blot, ICC/IF, Flow cytometry, ELISA에 적합하며, 화학적 반응성 물질 연구에 유용합니다. 단백질 G로 정제된 액상 형태로 4°C에서 보관합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50 |
| Flow Cytometry (Flow) | 1:100 |
| ELISA | 1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 6H6 |
| Immunogen | Synthetic Malondialdehyde modified Keyhole Limpet Hemocyanin (KLH) |
| Conjugate | PE (R-Phycoerythrin) |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932258 |
Additional Formats
- Unconjugated (MA5-27559)
- APC (MA5-45801)
- FITC (MA5-45802)
- PerCP (MA5-45803)
- Custom conjugation available upon request
Product Specific Information
A 1:1,000 dilution of MA5-45804 was sufficient for detection of Malondialdehyde in 2 µg of Malondialdehyde conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
Specific for Malondialdehyde-conjugated proteins. Does not detect free Malondialdehyde. Does not cross-react with Acrolein, Crotonaldehyde, Hexanoyl Lysine, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, or Methylglyoxal modified proteins.
Target Information
Malondialdehyde (CH₂(CHO)₂) is an organic compound and a byproduct of lipid metabolism. It is a highly reactive electrophile species that contributes to toxic stress in cells by forming covalent protein adducts (advanced lipoxidation end products, ALE). It can also form mutagenic DNA adducts with deoxyadenosine and deoxyguanosine. Malondialdehyde is present in many foods and found in high levels in rancid products.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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