Thermo Fisher Scientific Malondialdehyde Monoclonal Antibody (11E3), APC
Malondialdehyde 단백질 결합체를 특이적으로 검출하는 APC 형광 접합 단일클론 항체입니다. Western blot, IHC, ICC/IF, ELISA 등 다양한 응용에 적합합니다. 화학적 반응종 연구 및 산화 스트레스 분석에 유용합니다. 마우스 IgG1 기반 단일클론 항체로 높은 특이성과 재현성을 제공합니다.
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Thermo Fisher Scientific Malondialdehyde Monoclonal Antibody (11E3), APC
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | 1:100 |
| Immunocytochemistry (ICC/IF) | 1:50 |
| ELISA | 1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 11E3 |
| Immunogen | Synthetic Malondialdehyde modified Keyhole Limpet Hemocyanin (KLH) |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.46 mM phosphate / 2.48 mM MES, pH 7.4, with 2 mM EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932259 |
Available Formats
- Unconjugated (Cat. MA5-27560)
- FITC (Cat. MA5-45806)
- PE (Cat. MA5-45808)
- PerCP (Cat. MA5-45807)
- Custom conjugation available upon request
Product Specific Information
A 1:1,000 dilution of MA5-45805 was sufficient for detection of Malondialdehyde in 2 µg of Malondialdehyde conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
Specific for Malondialdehyde conjugated proteins. Does not detect free Malondialdehyde.
No cross-reactivity with Acrolein, Crotonaldehyde, Hexanoyl Lysine, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, or Methylglyoxal modified proteins.
Target Information
Malondialdehyde (CH₂(CHO)₂) is a byproduct of lipid metabolism and a reactive electrophile species that induces cellular toxic stress. It forms covalent protein adducts known as advanced lipoxidation end products (ALE) and mutagenic DNA adducts with deoxyadenosine and deoxyguanosine.
Malondialdehyde is also found in various foods, with elevated levels in rancid items.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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