Thermo Fisher Scientific HLA-DR Monoclonal Antibody (LN3), Alexa Fluor 660, eBioscience

Thermo Fisher Scientific HLA-DR Monoclonal Antibody (LN3), Alexa Fluor 660, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.125 µg)/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• Alexa Fluor Plus 488
• Alexa Fluor Plus 647
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395 / 496 / 563 / 615 / 661 / 737 / 805
• Brilliant Violet 421 / 480 / 605 / 711 / 786
• eFluor 450 / 506
• FITC
• NovaFluor Blue 510 / 530 / 555 / 610-30S / 660-40S / 725 / 800
• NovaFluor UV 765 / Violet 690 / Violet 800 / Yellow 810
• PE / PE-Cyanine5 / PE-Cyanine7 / PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436 / 600 / 645 / 702 / 780
• Request custom conjugation available

Product Specific Information

The LN3 monoclonal antibody reacts with human MHC class II molecule HLA-DR, expressed on antigen-presenting cells (APCs) such as B cells, monocytes, macrophages, dendritic cells, and activated T cells.
HLA-DR is a heterodimeric transmembrane protein composed of alpha and beta subunits, crucial for peptide presentation to CD4+ T lymphocytes.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-diluted and validated for flow cytometry of normal human peripheral blood cells
• Recommended Usage: 5 µL (0.125 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test)

Alexa Fluor 660 emits at 690 nm and is suitable for spectral cytometers, multiplexing with APC, Alexa Fluor 647, and Alexa Fluor 700.

• Excitation: 662 nm
• Emission: 690 nm
• Laser: Red Laser

Target Information

HLA-DR is a transmembrane glycoprotein composed of a 36 kDa alpha chain (DRA) and a 27 kDa beta chain (DRB).
The alpha chain gene (DRA) contains five exons; exons 2 and 3 encode extracellular domains. DRA serves as the sole alpha chain for DRB1, DRB3, DRB4, and DRB5.
DRB polymorphisms define peptide binding specificities, with hundreds of alleles identified—important for transplantation typing.

HLA-DR is primarily expressed on antigen-presenting cells such as B lymphocytes, monocytes, macrophages, thymic epithelial cells, and activated T lymphocytes.
MHC class II molecules bind intracellular peptides and present them to T-helper cells, initiating immune responses.
HLA and MHC antibodies are vital tools in Immunopeptidomics, supporting neoantigen identification via LC-MS/MS.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지