Thermo Fisher Scientific HIC1 Monoclonal Antibody (1F2)

Applications

Western Blot (WB)

• Tested Dilution: 1–5 µg/mL

ELISA

• Tested Dilution: 10 µg/mL

Product Specifications

Product Specific Information

Sequence of this protein is as follows:
PEGVFAVARL TAEQLSLKQQ DKAAAAELLA QTTHFLHDPK VALESLYPLA KFTAELGLSP DKAAEVLSQG AHLAAGPDG

Target Information

Hypermethylated in cancer (HIC-1) was originally identified as a target of p53-induced gene expression.
HIC-1 is deleted in the genetic disorder Miller-Dieker syndrome (MDS), and its expression is frequently suppressed in leukemia and various cancers due to hypermethylation of specific DNA regions leading to transcriptional silencing.

HIC-1 acts as a putative tumor suppressor protein that mediates transcriptional repression. It is ubiquitously expressed in adult tissues and contains five zinc fingers and an N-terminal broad complex POZ (BTB) domain.

In BTB/POZ containing proteins such as BCL-6 and PLZF, this domain interacts with the SMRT/N-CoR-mSin3A HDAC complex and represses gene transcription. When deleted (e.g., PLZF-RAR chimera in leukemias), transcriptional repression is attenuated.
Conversely, HIC-1 does not interact with HDAC complex components, suggesting HIC-1-induced transcriptional repression is independent of the POZ/BTB domain.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.