Thermo Fisher Scientific PI4K alpha, type I Polyclonal Antibody
PI4K alpha 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ELISA, IP 등 다양한 응용에 적합합니다. 인간 유래 단백질에 반응하며, 고순도 친화 크로마토그래피로 정제되었습니다. 연구용으로만 사용됩니다.
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Thermo Fisher Scientific PI4K alpha, type I Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5 µg/mL |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to an internal region of human PI4K, type I alpha protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.75 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 30% glycerol |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge if not clear after standing at room temperature. Product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
A BLAST analysis suggests cross-reactivity with mouse and human based on 100% sequence homology. Cross-reactivity with PI4K, type I alpha from other sources has not been determined.
Target Information
PIP5K1A lipid kinase (phosphatidylinositol-4-phosphate 5-kinase 1 alpha) phosphorylates phosphatidylinositol-4-phosphate to form phosphatidylinositol 4,5-bisphosphate. It is required for Wnt3a-induced LRP6 phosphorylation at Ser1490 in mammalian cells. Wnt3a stimulates phosphatidylinositol 4,5-bisphosphate formation through frizzled and disheveled, which directly interact with and activate PIP5KI. PIP5K1A-mediated PIP(2) production is crucial for HIV-1 entry and early infection steps in permissive lymphocytes. Overexpression of wild-type PI4P5K1A increases HIV-1 Env-mediated PIP(2) production and enhances viral replication in primary lymphocytes.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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