Thermo Fisher Scientific PI4K alpha, type I Polyclonal Antibody

Thermo Fisher Scientific PI4K alpha, type I Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge if not clear after standing at room temperature. Product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.

A BLAST analysis suggests cross-reactivity with mouse and human based on 100% sequence homology. Cross-reactivity with PI4K, type I alpha from other sources has not been determined.

Target Information

PIP5K1A lipid kinase (phosphatidylinositol-4-phosphate 5-kinase 1 alpha) phosphorylates phosphatidylinositol-4-phosphate to form phosphatidylinositol 4,5-bisphosphate. It is required for Wnt3a-induced LRP6 phosphorylation at Ser1490 in mammalian cells. Wnt3a stimulates phosphatidylinositol 4,5-bisphosphate formation through frizzled and disheveled, which directly interact with and activate PIP5KI. PIP5K1A-mediated PIP(2) production is crucial for HIV-1 entry and early infection steps in permissive lymphocytes. Overexpression of wild-type PI4P5K1A increases HIV-1 Env-mediated PIP(2) production and enhances viral replication in primary lymphocytes.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.