Thermo Fisher Scientific PI4KII alpha Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

-

Immunohistochemistry (IHC)

Assay-dependent

-

ELISA (ELISA)

1: 10,000

-

Immunoprecipitation (IP)

1 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Anti-PI4KII alpha affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide at the N-terminal of human P4K2A protein. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PI4K2A,uniProtId:Q9BTU6-1,ncbiNodeId:9606,antigenRange:1,antigenLength:479,antigenImageFileName:600-401-J93_PI4K2A_Q9BTU6-1_Rabbit.svg,antigenImageFileNamePDP:600-401-J93_PI4K2A_Q9BTU6-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.31 mg/mL

Purification

Affinity chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 50% glycerol, 0.15M NaCl

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

A BLAST analysis was used to suggest cross-reactivity with human, mouse, and rat based on 100% sequence homology. Cross-reactivity with PI4KII alpha from other sources has not been determined.

Target Information

PI4K2A (PI4K2 alpha) lipid kinase or phosphatidylinositol 4-kinase type 2 alpha is involved in the formation of phosphatidylinositolpolyphosphates (PtdInsPs) which are centrally involved in many biologic processes ranging from cell growth and organization of the actin cytoskeleton to endo- and exocytosis. PI4K2A phosphorylates PtdIns at the D-4 position which is an essential step in the biosynthesis of PtdInsPs. PI4K2A is a novel regulator of tumor growth by its action on angiogenesis and HIF-1alpha regulation. PI4K2A generates PtdIns4P-rich domains within the Golgi which regulate targeting of clathrin adaptor AP-1 complexes. PI4K2A is also required for Wnt to regulate LRP6 phosphorylation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.