Thermo Fisher Scientific Phospho-Elk1 (Ser383) Polyclonal Antibody
Phospho-Elk1 (Ser383) 폴리클로날 항체로, ELK1 단백질의 Ser383 인산화 부위를 특이적으로 인식합니다. Western blot, IHC, ICC/IF, IP에 사용 가능하며, 인간, 마우스, 원숭이, 랫트 시료에 반응합니다. PBS 버퍼와 글리세롤 기반의 액상 형태로 제공됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence (ICC/IF) | 1:100–1:500 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human ELK1 (Accession P19419), corresponding to amino acid residues around phosphorylated Ser383 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816305 |
Product Specific Information
This antibody detects endogenous levels of ELK1 only when phosphorylated at Serine 383.
Target Information
ELK1 is a component of the ternary complex that binds the serum response element (SRE) and mediates gene activity in response to serum and growth factors. It is phosphorylated by MAP kinase pathways at a cluster of serine/threonine motifs at its C-terminus. Phosphorylation at these sites, particularly Ser383, is critical for transcriptional activation by ELK1. ELK1 serves as a direct target of activated MAP kinase. Studies indicate ELK1 is a good substrate for MAP kinase, and its phosphorylation correlates with MAP kinase activity. Mutants of MAP kinase can block ELK1 activation in vivo. ELK1 acts as a nuclear target for the Ras-Raf-MAPK signaling cascade. Alternatively spliced transcript variants encoding the same protein have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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