Thermo Fisher Scientific HLA-B Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:3,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:100–1:1,000

Immunocytochemistry (ICC/IF)

• Tested Concentration: 5 µg/mL

Product Specifications

Product Specific Information

• Recommended positive control: HL-60
• Predicted reactivity: Human (94%)
• Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

HLA-B belongs to the HLA class I heavy chain paralogues. This molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is membrane-anchored and plays a central role in presenting peptides derived from the endoplasmic reticulum lumen to the immune system.
It is expressed in nearly all cells, with the heavy chain approximately 45 kDa in size and the gene comprising 8 exons. Exon 1 encodes the leader peptide; exons 2 and 3 encode the alpha1 and alpha2 domains, which bind peptides; exon 4 encodes the alpha3 domain; exon 5 encodes the transmembrane region; and exons 6–7 encode the cytoplasmic tail.
Polymorphisms within exons 2 and 3 determine peptide binding specificity, important for transplantation typing. Hundreds of HLA-B alleles have been described.

HLA and MHC antibodies are critical tools in immunopeptidomics, enabling identification and characterization of neoantigens via HPLC coupled to tandem mass spectrometry.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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