Thermo Fisher Scientific NBS1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:200

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

Flow Cytometry (Flow)

Assay-Dependent

-

ELISA (ELISA)

Assay-Dependent

-

Immunoprecipitation (IP)

3 µL

-

ChIP assay (ChIP)

Assay-Dependent

-

Product Specifications

Species Reactivity

Hamster, Human, Mammal, Mouse, Non-human primate

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Partial length human NBS1 protein [Swiss-Prot #O60934]

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum

Contains

0.02% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Wet ice

RRID

AB_2149256

Product Specific Information

ICC/IF has been performed using methanol-fixed IMR90 primary human fibroblasts.

Suggested positive control: antigen standard for NBN (transient overexpression lysate), TK6 and HeLa whole cell extract.

Target Information

The p95 gene (identical to NBS1 and nibrin) is a member of the hMre11/hRad50 double-strand break complex. This protein complex has been implicated in Nijmegen breakage syndrome, an autosomal recessive disorder marked by increased cancer incidence, cell cycle checkpoint deficits, and ionizing radiation sensitivity, thus revealing a direct molecular link between double-strand break repair and cell cycle checkpoint functions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.