Thermo Fisher Scientific HIF-1 beta Polyclonal Antibody
HIF-1 beta 단백질 검출용 Rabbit Polyclonal Antibody로, WB, IHC, ICC, IP, ChIP 등 다양한 응용에 적합합니다. Human 등 여러 종에 반응하며, hypoxia 관련 질환 연구에 활용됩니다. 액상 형태로 -20°C 이하 보관 권장.
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Thermo Fisher Scientific HIF-1 beta Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | Assay-Dependent |
| Immunoprecipitation (IP) | 1:10–1:500 |
| ChIP assay (ChIP) | 1:10–1:500 |
| Gel Shift (GS) | Assay-Dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Dog, Fish, Ferret, Human, Mouse, Sheep, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Fusion protein to HIF-1 beta containing residues 496–789 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not Determined |
| Storage Buffer | Whole serum |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Wet ice |
| RRID | AB_2274293 |
Product Specific Information
- In Western blot, background may increase with whole cell extract preparations.
- Lower dilutions may be needed for whole cell extract preps.
- Suggested positive controls: MCF-7 human nuclear extracts, MCF-7 whole cell lysate, antigen standard for ARNT (transient overexpression lysate).
Target Information
HIF-1 beta is one of the aryl hydrocarbon receptor nuclear translocator (ARNT) gene products.
Hypoxia plays a significant role in the pathophysiology of major human diseases such as myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease, and chronic obstructive pulmonary disease.
HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis through posttranslational modification by prolyl hydroxylation. It forms a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits.
Under normoxic conditions, HIF-1 alpha undergoes hydroxylation, ubiquitination, and degradation. Under hypoxic conditions, hydroxylation is inhibited, leading to increased transcriptional activity and accumulation of HIF-1 alpha.
In contrast, HIF-1 beta remains stable regardless of oxygen conditions.
Dysfunction of HIF-1 beta is associated with hypoxia and renal cell carcinoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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