Thermo Fisher Scientific HIF-1 beta Polyclonal Antibody

Thermo Fisher Scientific HIF-1 beta Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

• In Western blot, background may increase with whole cell extract preparations.
• Lower dilutions may be needed for whole cell extract preps.
• Suggested positive controls: MCF-7 human nuclear extracts, MCF-7 whole cell lysate, antigen standard for ARNT (transient overexpression lysate).

Target Information

HIF-1 beta is one of the aryl hydrocarbon receptor nuclear translocator (ARNT) gene products.
Hypoxia plays a significant role in the pathophysiology of major human diseases such as myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease, and chronic obstructive pulmonary disease.

HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis through posttranslational modification by prolyl hydroxylation. It forms a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits.
Under normoxic conditions, HIF-1 alpha undergoes hydroxylation, ubiquitination, and degradation. Under hypoxic conditions, hydroxylation is inhibited, leading to increased transcriptional activity and accumulation of HIF-1 alpha.
In contrast, HIF-1 beta remains stable regardless of oxygen conditions.
Dysfunction of HIF-1 beta is associated with hypoxia and renal cell carcinoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.