Thermo Fisher Scientific BTRC Polyclonal Antibody
BTRC 단백질을 인식하는 Thermo Fisher Scientific의 폴리클로날 항체로, Western blot, IHC, ICC/IF, ELISA에 사용 가능. 인간, 마우스, 랫트 반응성. Rabbit IgG 기반으로 친화 크로마토그래피 정제. -20°C 보관, 동결/해동 반복 피할 것.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1–120 of human β-TrCP/BTRC (NP_3786631) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.83 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | –20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2854858 |
Product Specific Information
Immunogen sequence:
MDPAEAVLQE KALKFMCSMP RSLWLGCSSL ADSMPSLRCL YNPGTGALTA FQNSSEREDC NNGEPPRKII PEKNSLRQTY NSCARLCLNQ ETVCLASTAM KTENCVAKTK LANGTSSMIV
Target Information
This gene encodes a member of the F-box protein family, characterized by an approximately 40 amino acid F-box motif. F-box proteins are subunits of the SCF ubiquitin ligase complex, functioning in phosphorylation-dependent ubiquitination. They are classified into three types:
- Fbws: Containing WD-40 domains
- Fbls: Containing leucine-rich repeats
- Fbxs: Containing other interaction modules or no recognizable motifs
The encoded protein belongs to the Fbws class and contains multiple WD-40 repeats. It is homologous to Xenopus bTrCP1, yeast Met30, Neurospora Scon2, and Drosophila Slimb proteins. It interacts with HIV-1 Vpu, connecting CD4 to the proteolytic machinery, and associates with phosphorylated IkappaBalpha and beta-catenin destruction motifs. It likely functions in transcriptional regulation by activating NF-kappaB and inhibiting beta-catenin pathways.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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