Thermo Fisher Scientific FceR1 alpha Monoclonal Antibody (MAR-1), PerCP-eFluor 710, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats Available

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• eFluor 450
• FITC
• Functional Grade
• PE
• PE-Cyanine7
• PE-eFluor 610
• Super Bright 436
• Super Bright 600
• Request Custom Conjugation

Product Specific Information

Description:
The MAR-1 monoclonal antibody reacts with the Fc epsilon Receptor I alpha subunit (FcεRIα), an IgE-binding subunit lacking signal-transducing ability. FcεRIα is expressed on mast and basophil cells and is up-regulated by the presence of IgE. It forms a tetrameric complex with one beta and two gamma subunits containing ITAM motifs, playing a key role in IgE-mediated allergic reactions.

Applications Reported:
Reported for use in flow cytometric analysis.

Applications Tested:
Tested by flow cytometric analysis of MC/9 mouse mast cell line. Recommended ≤0.06 µg per test (100 µL final volume). Optimize antibody concentration empirically for best performance.

Fluorochrome Information:
PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Recommended filter: 710/50 bandpass (695/40 acceptable alternative). Ensure instrument compatibility.

Fixation:
Samples may be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-54) for up to 3 days at 4°C in the dark. Clone-specific performance should be verified empirically.

Excitation / Emission / Laser:
488 nm / 710 nm / Blue Laser

Filtration:
0.2 µm post-manufacturing filtered.

Target Information

FceR1 (Fc epsilonR1 alpha) is a subunit of the high-affinity receptor for IgE. It forms a tetrameric complex (α, β, and two γ subunits), with β and γ responsible for signal transduction. This receptor mediates allergic responses by triggering histamine release upon allergen-bound IgE crosslinking. It plays a central role in allergic disorders such as hay fever and asthma through activation of mast cells and basophils.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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