Thermo Fisher Scientific IL-5 Polyclonal Antibody
고양이 IL-5에 반응하는 Thermo Fisher Scientific의 goat polyclonal antibody로, WB, ICC/IF, Neutralization 등에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, 보존제 무첨가 PBS buffer에 동결건조 형태로 제공. 연구용으로만 사용.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1 µg/mL |
| Immunocytochemistry (ICC/IF) | 5–15 µg/mL |
| Neutralization (Neu) | 0.5–2.0 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Cat |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant feline IL-5 (Ile20–Ser134) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No Preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2609991 |
Product Specific Information
- In direct ELISAs, approximately 80% cross-reactivity with recombinant bovine IL-5 and recombinant equine IL-5 is observed.
- Approximately 50% cross-reactivity with recombinant porcine IL-5 and recombinant canine IL-5 is observed.
- Approximately 10% cross-reactivity with recombinant human IL-5 is observed.
- Less than 1% cross-reactivity with recombinant rat IL-5, recombinant mouse IL-5, and recombinant rhesus monkey IL-5 is observed.
- Reconstitute at 0.2 mg/mL in sterile PBS.
- Endotoxin level: <0.10 EU per 1 µg of antibody (LAL method).
Target Information
IL-5 is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. It is a key regulator of eosinopoiesis, eosinophil maturation, and activation. Elevated IL-5 production is associated with asthma and hypereosinophilic syndromes.
The IL-5 receptor is a heterodimer whose beta subunit is shared with IL3 and CSF2 (GM-CSF) receptors. IL-5, IL4, and IL13 form a cytokine gene cluster on chromosome 5q31 and are coordinately regulated by long-range regulatory elements.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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