Thermo Fisher Scientific NLE1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human NLE1. Recombinant protein control fragment (Product #RP-92396). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:NLE1,uniProtId:Q9NVX2-1,ncbiNodeId:9606,antigenRange:268-353,antigenLength:485,antigenImageFileName:PA5-53850_NLE1_Q9NVX2-1_Rabbit.svg,antigenImageFileNamePDP:PA5-53850_NLE1_Q9NVX2-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2644710

Product Specific Information

Immunogen sequence: TIKVWRAHDG VLCRTLQGHG HWVNTMALST DYALRTGAFE PAEASVNPQD LQGSLQELKE RALSRYNLVR GQGPERLVSG SDDFTL

Highest antigen sequence identity to the following orthologs: Mouse - 95%, Rat - 95%.

Target Information

NLE1 plays a role in regulating Notch activity, in regulating the expression of CDKN1A and several members of the Wnt pathway, probably via its effects on Notch activity. NLE1 is required during embryogenesis for inner mass cell survival.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.