Thermo Fisher Scientific Phospho-AKT1 (Ser473) Polyclonal Antibody
Phospho-AKT1 (Ser473) 항체는 인간, 마우스, 랫트에 반응하는 Rabbit Polyclonal 항체입니다. Western blot, IHC, ICC, IP에 사용 가능하며, AKT1 인산화 상태 분석에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 장기 보관 시 -20°C에서 안정합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | - |
| Immunoprecipitation (IP) | 1:100–1:500 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Carrier-protein conjugated synthetic peptide corresponding to residues around human AKT (phospho Ser473/474/472) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol, 1% BSA |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C (short term). For long-term storage, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2792653 |
Product Specific Information
- Keep as a concentrated solution.
- Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (100%), Xenopus laevis (100%), Cat (100%), Pig (100%), Chicken (100%), Sheep (100%), Rhesus Monkey (100%).
- Positive Controls: PC-12, Rat2, MCF-7 (with PMA treatments for 1–6 hr), HUVEC (±VEGF), NIH-3T3 (±PDGF).
- Centrifuge briefly before opening the vial.
Target Information
AKT1 (PKB alpha) is a serine/threonine kinase that regulates cell survival.
Activated AKT1 inhibits apoptosis and promotes cell cycle progression by phosphorylating multiple substrates in various cell types, including cancer cells.
It is activated by insulin, PI3K, IGF1, and other growth factors.
The AKT pathway is a major target for cancer drug discovery due to its critical role in cell survival and proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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