Thermo Fisher Scientific 5-Methylcytosine Polyclonal Antibody

Applications

Tested Dilution

Publications

Dot blot (DB)

Assay-dependent

-

Immunomicroscopy (IM)

Assay-dependent

-

Methylated DNA Immunoprecipitation (MeDIP)

Assay-dependent

-

Product Specifications

Species Reactivity

Chemical

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

The immunogen used to generate this antibody corresponds to 5-MethylCytosine/ 5-mC

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

7.55 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2792656

Product Specific Information

Keep as concentrated solution.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Methylation of DNA cytosine bases plays important roles in the regulation of gene transcription. It has been estimated that 60 to 90% of the cytosines in CpG dinucleotides are methylated. An increase in methyl-CpG correlates with transcriptional silencing for the whole chromosome especially in developmentally regulated genes. Methylation is believed to lead to transcriptional silencing by multiple mechanisms including alteration of transcription factor binding to promoters and alteration in chromatin structure.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.