Thermo Fisher Scientific Phospho-RAD17 (Ser86) Polyclonal Antibody
RAD17 단백질의 Ser86 인산화 형태를 특이적으로 인식하는 Rabbit Polyclonal Antibody. Western blot, IHC, ICC/IF 실험에 적합. 인간, 생쥐, 랫드 반응성. 고순도 액상 형태로 제공되며 -20°C에서 안정적으로 보관 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthesized peptide derived from human RAD17 (Accession O75943), corresponding to amino acid residues around phosphorylated Ser86 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816863 |
Product Specific Information
This antibody detects endogenous levels of RAD17 only when phosphorylated at Ser86.
Target Information
Rad17 is essential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage.
It has weak ATPase activity required for chromatin binding and participates in recruitment of the RAD1-RAD9-HUS1 complex and RHNO1 onto chromatin, as well as in CHEK1 activation.
Rad17 may also serve as a sensor of DNA replication progression and be involved in homologous recombination.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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