Thermo Fisher Scientific CD8a Monoclonal Antibody (SK1), PE, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.125 µg)/test
• Publications: View 10 publications

Product Specifications

Available Formats

• Alexa Fluor 700
• APC
• APC-eFluor 780
• Brilliant UV 805
• Brilliant Violet 650
• eFluor 450
• FITC
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Yellow 730
• NovaFluor Yellow 755
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

The SK1 monoclonal antibody reacts with the human CD8a molecule, an approximately 32–34 kDa cell surface receptor expressed as a heterodimer with the CD8 beta chain (CD8α/β) or as a homodimer (CD8α/α). CD8a is expressed on most thymocytes and a subset of mature T cells and NK cells. CD8 binds to MHC class I and, through association with protein tyrosine kinase p56lck, plays a key role in T-cell development and activation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-titrated and validated for human peripheral blood cells
• Recommended Use: 5 µL (0.125 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test)
• Excitation: 488–561 nm
• Emission: 578 nm
• Compatible Lasers: Blue, Green, Yellow-Green
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

Cluster of differentiation 8 (CD8) is a type I transmembrane glycoprotein of the immunoglobulin family involved in T cell signal transduction, differentiation, and activation. CD8 is mainly expressed on T cells as a disulfide-linked heterodimer of CD8α and CD8β, functioning as a co-receptor with TCR for MHC class I molecules. The CD8α chain is essential for MHC-I binding and is also expressed as a homodimer on subsets of T cells, NK cells, monocytes, and dendritic cells. Ligation of MHC-I/peptide complexes triggers recruitment of protein tyrosine kinase Lck, leading to lymphokine production and cytotoxic T lymphocyte (CTL) activation. Activated CTLs are crucial for pathogen and tumor cell clearance, and their differentiation is enhanced by IL-2, IL-12, and TGF-β1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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