Thermo Fisher Scientific HRK Polyclonal Antibody
HRK 단백질을 인식하는 Rabbit Polyclonal Antibody로 Western Blot에 적합. 인간 및 생쥐 시료에 반응하며, 항원 친화 크로마토그래피로 정제됨. 액상 형태로 제공되며, 단기 냉장·장기 냉동 보관 가능. 세포자멸사 연구에 유용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 2.5–5 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A 15 amino acid peptide from near the center of human Hrk |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Maintain refrigerated at 2–8°C for up to 3 months. For long term storage store at -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11153786 |
Product Specific Information
Despite its predicted molecular weight, Hrk often migrates at 12–15 kDa in SDS-PAGE.
A suggested positive control is mouse pancreas tissue lysate.
PA5-20235 can be used with blocking peptide PEP-0369.
Target Information
Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells.
Hrk, a pro-apoptotic member of the Bcl-2 homology domain-3 (BH3)-only group of the Bcl-2 family of proteins, is a novel protein induced during programmed neuronal death.
It lacks significant homology to other Bcl-2 family members except for an 8-amino acid region similar to the BH3 motif of Bik.
Hrk regulates apoptosis through interaction with anti-apoptotic proteins Bcl-2 and Bcl-XL via this domain, but does not interact with pro-apoptotic proteins Bax, Bak, or Bcl-XS.
Hrk localizes to mitochondrial membranes in a pattern similar to that of Bcl-2 and Bcl-XL.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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