Thermo Fisher Scientific LexA Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:3,000

-

Immunohistochemistry (IHC)

Assay-dependent

-

Immunoprecipitation (IP)

Assay-dependent

-

ChIP assay (ChIP)

Assay-dependent

-

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bacteria, Yeast

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Full size LexA recombinant protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_667485

Product Specific Information

PA1-4966 detects LexA protein in E.coli and yeast samples.

PA1-4966 has been successfully used in Western blot, immunoprecipitation and immunohistochemical procedures. It is specific for the ~23 kDa LexA protein.

PA1-4966 immunogen is a highly purified recombinant full size LexA protein.

Store at -20ºC.

Target Information

E. coli LexA protein inhibits the transcription of the genes belonging to the SOS regulon that are related to DNA repair and cell division by recognizing and binding to the SOS-box sequence. LexA‘s self-protease activity is promoted by RecA protein which, responding to DNA damage, is activated by its binding to single-strand DNA accumulated in the cells. It is cleaved into two fragments and loses its function as a repressor. As the result, the expression of genes belonging to the SOS regulon is induced, and mutagenic activity in the cells are enhanced.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.