Thermo Fisher Scientific HIV1 nef Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:3,000

-

ELISA (ELISA)

Assay-dependent

-

Immunoprecipitation (IP)

Assay-dependent

-

Dot blot (DB)

1:3,000

-

Product Specifications

Species Reactivity

Virus

Host/Isotype

Guinea pig / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Purified full-size recombinant Nef of HIV1 group M, subtype B expressed in E. coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum

Contains

0.09% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_612267

Product Specific Information

PA1-4963 detects the HIV-1 Nef protein in HIV samples.

PA1-4963 has been successfully used in Western blot and ELISA procedures. By Western blot this antibody detects a ~27 kDa protein, corresponding to HIV-1 Nef in HIV samples.

PA1-4963 immunogen corresponds to the recombinant protein which was over-expressed in E. coli with a plasmid carrying the Nef coding region of the HIV-1 virus.

Recombinant HIV-1 Nef protein (Cat. # RP-4922 or RP-4923) can be purchased for control experiments in Western blot assays.

Target Information

HIV-1 Nef is one of the accessory proteins synthesized in the early stage of AIDS virus reproduction and is abundantly found in infected cells. The name derives from its negative factor thought at the beginning but presently it is remarked as the protein which bears a most distinctive biological characteristic of AIDS virus. The protein interacts directly with the signal transduction protein of the host T cell and works effectively on AIDS infection or on long term survival of the infected cells or induces apoptosis of non-infected cells. It is also involved in the endocytosis and degradation of receptor protein of the cell surface such as CD 4 and MH4, important for AIDS virus infection.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.