Thermo Fisher Scientific NUR77 Recombinant Rabbit Monoclonal Antibody (JM59-11)
NUR77 단백질을 표적으로 하는 Recombinant Rabbit Monoclonal Antibody(JM59-11). Western blot, IHC, ICC/IF, Flow cytometry에 사용 가능. 높은 특이성과 일관된 로트 간 재현성 제공. 단백질 A 정제, 1 mg/mL 농도, 4°C 또는 -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:2,000–1:5,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 | - |
| Flow Cytometry (Flow) | 1:1,000 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JM59-11 |
| Immunogen | Synthetic peptide within Human NUR77 (aa 10–49) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% Sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809924 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned, and individual clones are screened to select optimal candidates.
Advantages:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broad immunoreactivity due to rabbit immune repertoire
Target Information
Target: NR4A1 (Nur77)
NR4A1 belongs to the steroid-thyroid hormone-retinoid receptor superfamily. It is expressed in brain, adrenal, thyroid, liver, testis, ovary, thymus, muscle, lung, and prostate. Expression is induced by stress stimuli and growth factors.
This nuclear transcription factor mediates apoptotic signaling in thymocytes and tumor cells and participates in hypothalamic-pituitary axis signaling. Translocation from nucleus to mitochondria triggers apoptosis.
Three transcript variants encoding two isoforms have been identified. Variant 3 (TRC beta) differs from variant 1, producing isoform b with a distinct C-terminus. RXR is known as a partner for NR4A1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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